Objective Utilizing Beer’s law, known concentrations of bovine serum albumin (BSA) are reacted with bicinchonic acid (BCA) to create a standard curve of absorbance vs. concentration of protein. This curve is then utilized to determine the concentration of protein in an unknown solution. Introduction Beer’s law () tells us that the light absorbance (A) of a solution is proportional to the concentration of the protein (c) within the solution, the light path (b) and the extinction coefficient of the protein. […]
