Enzyme Kinetics- Determination of the Kinetic Parameters for Tyrosinase By: Trevor Frisby & David Darby Abstract In this Kinetics laboratory experiment the enzyme tyrosinase was investigated in the presence of two types of inhibitors: sodium cyanide and a synthesized inhibitor, dimethoxy azo-stilbene. Tyrosinase, also known as monophenol monooxygenase utilizes oxygen for the catalysis of two separate reactions that involve the hydroxylation of monophenols to diphenols, and also the oxidative conversion of diphenols to quinones. Through these chemical pathways tyrosinase catalytically […]
