Fluorescence Spectrophotometry of Riboflavin By: Andrew Tam Introduction This experiment was done to explore an aspect of spectroscopic analysis called fluorescence. This aspect involves shining an ultraviolet light, which will excite the electrons of certain molecules. When these excited electrons returned to their ground (original) state, energy is released and causes the molecule to emit light. This light can then be captured with the use of a detector to measure how intense the compound emits light as a result of […]
