Quantitative Assay for Fluorescein By: David Gould In this experiment several concentrations and variations of fluorescein were created and measured spectrophotometrically to determine absorbance at a peak wavelength. A calibration curve was made from the external standards to ensure the data showed a trend, as well as get a rough idea of any concentrations from future absorbance readings. Once standard additions were prepared and a graph made from this data, a reliable concentration was also obtained using the x-intercept. Given […]
