Written by Brandon Ritter What does it mean to “work up” an organic reaction? Purpose: The purpose of this lab was to separate benzoic acid, 4-choloraniline and cholesterol using extraction. Reaction Equations: Missing 🙁 Structures: Calculations: Missing 🙁 Results: Benzoic Acid: Melting Point: 121.9-123.1⁰C Mass: 0.369g 4-chloroaniline: Melting Point: 68.6-70.2⁰C Mass: 0.043g Cholesterol: Melting Point: 146.7-147.2⁰C Mass: 0.205g Discussion: Our percent recovery for benzoic acid was really good, we got a lot more recovered benzoic acid product than expected. We got slightly […]
Written by Aida Martinez Introduction All organisms require a metabolic process that allows them to convert something they intake into energy. To convert light into energy, all organisms contain tetrapyrroles (involved in energy transfer, catalysis and transduction) (Reinbothe 1995). Of the greatest sources of energy is photosynthesis. Photosynthesis is a metabolic process (present in photosynthetic organisms) that intakes light, carbon dioxide, minerals, and water to produce chemical energy such as sugars, ATP and oxygen. Sugars and ATP are stored in […]
The absorption spectras of the five different solutions all differ in max wavelengths and ranges of wavelengths. The blue food coloring had one peak with a max absorption of .1201 at 630.7nm. The 1:1 mix of blue and yellow food coloring had two peaks with a max absorption of .1911 at 421.1nm. The yellow food coloring has one peak with a max absorbance of .3734 at 422.9nm. Type of Solution Color of Solution lmax values Blue Food Coloring Blue 630.7 […]
Written by Diane Krehbiel Abstract: In this experiment, two different methods were used to discover the acetic acid content in a sample of vinegar. The known percent of acetic acid in vinegar is 5%. The first method used was titrating with a visual indicator. The percent of acid found using this method was 5.086%. The second method was the use of pH meter to measure the change in pH. The percentage from using this method was 5.049%. The pH meter method […]
Written by Erin Downey INTRODUCTION A section of cloned DNA can be sequenced by a variety of methods, the most common of which is the Sanger method. The Sanger method uses low concentrations of dideoxynucleoside triphosphates (ddNTPs) to act as chain terminators. These are used with the normal, unmodified deoxynucleoside triphosphates. Terminators are separated into ddATP, ddTTPs, ddCTPs, and ddGTPs samples so that when they are sequenced, each lane identifies the terminal nucleotide. This results in fragments of varying […]
Written by: Nathan Sheppard Abstract Plasmids are pieces of circular DNA that are in bacteria which can code for genes that can cause the bacteria to have extra “features”. With the pGLO plasmid this extra “feature” causes bacteria to glow (from the Green Fluorescent Protein that was inserted) and also has resistance to ampicillin (from the beta-lactamase protein). By adding the pGLO plasmid the E.coli bacteria will be able to grow on agar with arabinose sugar and ampicillin. The […]
