Biginelli Reaction By: Mark Roberto Postlab Questions Questions 1, 3, and 4 on separate paper. 2) 3,4-dihydropyrimidones are calcium channel blockers. This means that they block calcium ion flow through membrane channels in cells.[1] Results Using the Bignelli reaction, acetoacetamide, benzaldehyde, and urea were reacted in a 1:1:1 ratio to synthesize a 3,4-dihydropyrimidinone that will be called “product”. The limiting reagent was determined to be the acetoacetamide, which was 3.936 mM (0.3979g/101.10g/mol). For the urea, 3.996 mM were used […]
Abstract The Cary UV-Visible Spectrophotometer varian was used to measure both NAD+ and NADH absorption from 600 nm to 220 nm in a 1 cm quartz cuvette. The max wavelength for absorption for NAD+ was 259 nm and 338 nm for NADH. Using beer’s law and the dilution factor of 200, the concentration for NADHwas 1.06 X 10-4 M in the cuvette and 0.02 M in the undiluted sample, while the NAD+ had a concentration of 2.58 X 10-5 M […]
Purpose: Using H-NMR, in this experiment we sought to study the effect of pH and polarity of the solvent on the Ktrans values of N-Acetyl-L-Proline. The Ktrans values are a means to examine the equilibrium between cis and trans isomers of N-Acetyl-L-Proline. The area under the peak of the H NMR was integrated and divided accordingly to give the Ktransvalues. Ktrans values were used in the calculation of the proportion of cis isomers in the sample. The picture below depicts […]
Give an experimental section for this laboratory experiment. BOTH WEEKS Week 1: Synthesis of a Nickel complex was determined by adding ethylenediamine and ethanol. After obtaining close to 1.000g of nickel(II) sulfate hexahydrate, with your partner as instructed in step one, we quantitatively transferred it to a 250mL beaker with no more than 3mL of water. After the substance had dissolved we added the assigned amount of en to the substance slowly so it could attach to the nickel around […]
Biochemical Activities By: Amanda Goldner Table of Contents Lab A: Media Preparation Lab 1: Inoculations, Smear Preps, and Simple Staining Lab 2: Gram Staining and Using the Spectrophotometer Lab 3: Negative, Acid Fast, Endospore, and Capsule Staining Lab 4: Biochemical Activities of Bacteria I ……………………………………….… 3 Lab 5: Bergey’s Manual (Practical) ………………………………………………… 11 Lab 6: Biochemical Activities of Bacteria II, Unknown Identification, and Rapid Multitests …………………………………………………………………………………………… 13 Lab 7: Isolation of Microorganisms From Soil Lab 8: Eukaryotes I: Fungi Lab […]
Heat Transfer – Pool Boiling in a Saturated Liquid By: Josh Lashbrook Abstract Experiment No. 5, Pool Boiling in a Saturate Liquid, was conducted in order to verify the boiling curve for nitrogen at 1atm, as well as walk through the steps of its original creation. The lab was done using liquid nitrogen and a two submerged copper spheres of different size. Nitrogen was used because of its extremely low boiling point. If water would have been used, time would […]
