Biochemical Activities By: Amanda Goldner Table of Contents Lab A: Media Preparation Lab 1: Inoculations, Smear Preps, and Simple Staining Lab 2: Gram Staining and Using the Spectrophotometer Lab 3: Negative, Acid Fast, Endospore, and Capsule Staining Lab 4: Biochemical Activities of Bacteria I ……………………………………….… 3 Lab 5: Bergey’s Manual (Practical) ………………………………………………… 11 Lab 6: Biochemical Activities of Bacteria II, Unknown Identification, and Rapid Multitests …………………………………………………………………………………………… 13 Lab 7: Isolation of Microorganisms From Soil Lab 8: Eukaryotes I: Fungi Lab […]
Heat Transfer – Pool Boiling in a Saturated Liquid By: Josh Lashbrook Abstract Experiment No. 5, Pool Boiling in a Saturate Liquid, was conducted in order to verify the boiling curve for nitrogen at 1atm, as well as walk through the steps of its original creation. The lab was done using liquid nitrogen and a two submerged copper spheres of different size. Nitrogen was used because of its extremely low boiling point. If water would have been used, time would […]
By: Colin Patrick, Trent McInturff, Michael Billups Abstract This report discusses the methods with which the viscosity of liquid glycerin is determined, and uses the data to validate Stokes’ Law. Two experiments were performed: the viscosity of glycerin was determined using a rotational viscometer, and the data used to validate Stokes’ Law was collected using a falling ball viscometer. Results showed that either method showed significant error and did not validate Stokes’ Law; though the revised Stokes’ Law equation gave […]
By: Ryan DeAngelis, Andrew Mound Introduction The purpose of this experiment is to synthesize an alcohol using hydroboration-oxidation and determine the regiochemistry and stereochemistry of the product via 1H-NMR, 13C-NMR, and Infrared spectroscopy.1 Hydroboration-Oxidation is a two-step process used to convert an alkene to an alcohol. The first step, hydroboration, is the addition of borane (BH3) to the alkene. It proceeds as a concerted mechanism with the electron pair in the pi bond attacking the electrophilic boron, and one of […]
Simple Distillation: Separation of Hexane and Toluene By: Jessica Hauger Abstract: The purpose of this experiment is to separate a mixture of hexane and toluene by collecting fractions through simple distillation. Because hexane’s boiling point is about 69°C and the boiling point of toluene is 111°C, the two compounds distill at different times. The distillation took place in a simple distilling apparatus. The refractive index was measured using an Abbé refractometer. After collecting only two fractions and recording the refractive […]
By: Erin H. ABSTRACT Phenylmagnesium bromide was used as the Grignard organometallic for two reactions. The first was a synthesis of triphenylmethanol from benzophenone. This was done using a gentle reflux followed by several Separatory washes and extractions to purify and separate product. The second reaction was the synthesis of benzoic acid from phenylmagnesium bromide using dry ice and a variety of washes. The benzoic acid reaction was straightforward and a 54.4% yield was recovered. The triphenylmethanol reaction was a […]
