Quantitative Assay for Fluorescein By: David Gould In this experiment several concentrations and variations of fluorescein were created and measured spectrophotometrically to determine absorbance at a peak wavelength. A calibration curve was made from the external standards to ensure the data showed a trend, as well as get a rough idea of any concentrations from future absorbance readings. Once standard additions were prepared and a graph made from this data, a reliable concentration was also obtained using the x-intercept. Given […]
The Synthesis and Gravimetric Analysis of Alum By: Omish Samaroo Introduction The purpose of this experiment is to synthesize the double salt alum, which has the formula KAl(SO4)2•nH2O, where n is the number of waters of crystallization that are incorporated into its crystalline structure. By determining the percent of water by mass in the compound through gravimetric analysis, the value of n in the empirical formula of alum can be determined. The presence of potassium, aluminum, and sulfate is determined […]
Electric Fields and Potentials By: Alexis Huddleston Introduction: The purpose of this experiment was to gain an understanding of electric fields and their potentials by using volts and point strategies. The electric potential as stated in this activity is created by the distribution of charges, which is a scalar quantity, determined by the different locations of voltage charges. Furthermore, potential charges are decreased when the points measured are farthest away from the equipotential base. Therefore, V is the electric […]
By: Ludmila Novikova Section A: Conductimetry and the Strength of Acids and Bases: Goal: To understand how conductivity experiments work and to be able to identify strong and weak acids and bases via the use of equilibrium constants. Summary: In this section of the lab, Dr. Thompson carried out several experiments to show how certain substances allow the conductivity of electricity to occur. 1.) The first experiment was one between sugars in water. There was a light bulb with a […]
By: Carla Mateo The main purpose of the experiment is to measure the rate of inversion of sucrose and its dependence on temperature. When discussing inversion, the origin of the usage of the term, in this context, comes from the way that the measurement is carried out. The sugar syrup concentration is measured using a polarimeter. The light, which is plane polarized, passes through a solution, a sample of pure sucrose, it undergoes a rotation. It is optically rotated to […]
Laboratory Manual Objectives. After completing this laboratory module, you should be able to: Understand, broadly, what occurs when drugs bind to blood serum proteins. Demonstrate proper use of the commercial UV/Vis spectrometer. Describe what happens to drug binding when concentration is varied. Use basic programming to upload and view a three dimensional protein structure. Introduction The most abundant amino acids in HSA are alanine (Ala), lysine (Lys), leucine (Leu), and glutamic acid (Glu).1,2 The abundance of the amino acids is […]
